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GPEx® Advantage: The High
Speed Solution |
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Using conventional gene insertion
and expression techniques, developing a stable production cell
line for a target protein can take as long as 18 months. GPEx® overcomes
the inefficiencies of conventional systems and delivers a stable
production cell line for your target protein in as little as
5-6 months.
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GPEx® generates stable cell
lines from the outset |
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Short Cut to Stable Cell Lines |
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The secret is the GPEx® retrovector technology
- a proprietary pseudotyped, high-titer vector which ensures
that stable transductions occur in virtually 100% of target cells
(CHO, 293). This eliminates the need for selectable markers,
saving time and cost. In contrast, conventional transfection
or electroporation methods can produce an insertion frequency
as low as 1 out of every 100,000 cells, necessitating additional
selection steps and selectable marker genes. They also carry
the risk of gene excision or silencing, making them relatively
unstable. Virtually any cDNA can be packaged into Gala’s
GPEx® retrovector and used
to infect your choice of mammalian cell lines. This technology
produces a stable
clonal cell line in about four months for proof of concept studies.
High producing
cell lines suitable for master cell banking and subsequent cGMP
production are
obtained less than six months after receipt of your cDNA. With
GPEx®, stable transduced cell lines are
produced from the outset, such that optimized cell lines can
be used for gram-scale production of your target
protein
in as little as 6 months after
we receive your cDNA. |
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